Effective test in laboratory
Bioassay results determined that the LD50 in 24h of 18% Hyperosmosis Omethoate to Aphids Rhopalosiohum pseudobrassicae Davis, Aphis gossypii Glov. and spider mite Tetranychus trunctus Ehara are 2.19~ 103, 1.9848~10-2 and 28.035~10-5ug/individule, which were 2.0, 3.4 and 3.8 times lower than that of 40% Omethoate. Contrast test in laboratory:
The control test in laboratory using potter spraying method and sheet immersion method (FAO, 1974) on resistant cotton spider mite, Tetranychus cinnabarmus (Boisduval) by Jiangsu Pesticide Institute as follows:
Tab.1. Omethoate+Azone action on cotton spider mite by spray method
Trials
Active content
(ppm)
Individuals
before test
Living individuals
after treatment
Efficiency
(%)
40% Omethoate+0.8% Azone
2.5
60
32
46.7
40% Omethoate+0.8% A.zone
5
59
8
86.4
40% Omethoate+1% Azone
2.5
62
55
11.3
40% Omethoate+1% Azone
5
64
7
89.1
40% Omethoate
5
60
35
41.7
40% Omethoate
10
59
4
93.2
CK (water)
-
32
32
0
Tab.2. Omethoate+Azone action on cotton spider mite by sheet immersion method
Trials
Diluting
times
Active coneent
(ppm)
Test
individuals
Dead individuals
in 24h
Mortality
(%)
200 Omethoate+O.8%,Azone
10000
20
76
33
43.42
20% Omethoate+0.8%Azone
5000
40
75
59
78.67
200 Omethoate+l%Azone
10000
20
78
32
41.03
20% Omethoate+l%oAzone
5000
40
74
43
58.11
40% Omethoate
10000
40
78
21
26.92
40% Omethoate
5000
80
79
39
49.37
The efficiency enhancing action of Azone to Omethoate:
The test result in Tab. 1 showed that only spraying Omethoate with 10ppm the control efficiency on the mites was 93.2% but using Omethoate adding 0.8% or 1% Azone the control efficiency were 86.4% and 89.1 respectively with 5 ppm. It suggested that the mixture with only a half active agent can achieve the nearly same control result than only using
Omethoate.
The test result in Tab.2 showed that the 10000 times dilution of 20% Omethoate with 0.8% and 1% Azone brout the spider mites' mortality by 43.42% and 41.03% respectively, which were 16.5% 14.15% respectively higher than that of 20% Omethoate 10000 times dilution (26.92%).Similarly, at the trials of 5000 times dilution, mites' mortality in Azone using trials were 78.67%, 58.11% respectively, which are higher than that in none Azone trial (49.37%) by 29.3% and 8.74% respectively. Among them,the trial adding 0.8% Azone had a statistic difference than that of none Azone trial at same active dosage.
The test results suggested that omethoate with 0.8-1% Azone can be used with only a half mount active agent to achieve the same efficiency.The efficiency enhancing action of Azone to Monocrotophos
The test results showed in table 3 and table 4 suggested that the efficiency enhancing function of Azone on Monocrotophos was similar to that on Omethoate. In spraying method tests the Azone dosage was used doubly than that in Omethoate which got more enhancing efficiency in the test. For example, 40% Monocrotophos 2ppm with 1.6% or 2% Azone had a control efficiency at 93% and 93.9% respectively, which higher than that in 4ppm with none Azone trail (84.4%) by 8.6% and 9.5% respectively. These results proved that adding 1.6-2% Azone Monocrotophos could get double efficiency at same dosage. In other words if Monocrotophos dosage decrease to half and add 0.8-1% Azone it can achieve the original efficiency also.
Tab.3.Monocrotophos+Azone contrast test result on cotton spider mite by spraying method
Trials
Active concent
(ppm)
Test
individuals
Living
individuals
Efficiency (%)
40% Monocrotophos+ 1.6%Azone
1
64
18
71.9
40% Monocrotophos+ 1.6%Azooe
2
57
4
93.0
40% Monocrotophos+2%Azone
1
59
9
84.7
40% Monocrotophos+2%Azone
2
66
4
93.9
40% Monocrotophos
2
55
16
70.9
40% Monoerotophos
4
64
10
84.4
CK (water)
-
62
50
19.4
Tab 4. Monocrotophos+Azone test results on cotton spider mite by sheet immersion method
TriMs
Dilution
times
Active concent
(ppm)
Test Dead
individuals
individuals
in 24h
Mortality
(%)
20%Monocrotophos+0.8% Azone
10000
20
74
52
70.27
20%oMonocrotophos+0.8% Azone
5000
40
97
73
75.26
20%oMonocrotophos+ 1% Azone
10000
20
73
55
75.34
20%Monocrotophos+l% Azone
5000
40
72
62
86.11
40%Monocrotophos
10000
40
77
53
68.83
40%,Monocrotophos
5000
80
92
81
88.04
Tab 5. Methamidophos+Azone test results on cotton spider mite by sheet immersion method
Trials
Dilution
times
Active content
(ppm)
Test Dead
individuals
individuals
in 24h
Mortality
(%)
25% Methamidophos +0.8% Azone
8000
31.25
75
28
37.33
25% Methamidophos +0.8% Azone
4000
62.5
80
63
78.75
25% Methamidophos+ 1% Azone
8000
31.25
80
33
41.25
25% Methamidophos+l% Azone
4000
62.5
79
51
64.56
50% Methamidophos
8000
62.5
77
18
23.38
50% Methamidophos
4000
125
78
29
37.18
The efficiency enhancing action of Azone to Methamidophos
Sheet immersion test results was showed in table 5. The results proved that Azone has a significant efficiency enhancing function to Methamidophos. The trials of 25% Methamidophos formulation adding 0.85% or 1% Azone had higher efficiency than that of 50% Methamidophos formulation in same diluting times solution, which were as much as 13.95%,17.87% in 8000 times dilution, and 41.57~/o, 27.38% in 4000 times dilution respectively higher than none Azone trials. Among them the trials with 0.8% Azone had statistical difference from none Azone trials in 4000 times dilution. The results suggested that adding Azone can reduce a half Methamidophos' dosage to achieve the same efficiency.
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